Document 0646
 DOCN  M9460646
 TI    Immunoglobulin class and subclass antibodies to HIV proteins in maternal
       serum: association with perinatal transmission.
 DT    9408
 AU    Mann DL; Hamlin-Green G; Willoughby A; Landesman SH; Goedert JJ;
       Immunogenetics Section, NCI-Frederick Cancer Research and; Development
       Center, Maryland 21702-1201.
 SO    J Acquir Immune Defic Syndr. 1994 Jun;7(6):617-22. Unique Identifier :
       AIDSLINE MED/94231467
 AB    A flow cytometry-based assay for detection of immunoglobulin (Ig) class
       and subclass antibodies in human serum or plasma was developed. With use
       of this procedure, the presence and relative frequency of antibody
       activity in the Ig classes and subclasses (IgA1, IgA2, IgD, IgE, IgG1,
       IgG2, IgG3, IgG4, and IgM) to human immunodeficiency virus type 1
       (HIV-1) proteins (gp160, gp120, p66, and p24) was determined in serum or
       plasma from a cohort of 47 HIV-1-infected, pregnant women. Antibody
       activity in each of the classes and subclasses was found with
       differences in frequency depending on the Ig class/subclass and the
       HIV-1 protein. IgG1 antibodies were the most frequently reactive Ig
       class/subclass to each protein. Intermediate frequencies of reactivity
       were found in IgA1, IgG2, IgG3, and IgM class and subclasses and
       antibodies of the IgA2, IgE, and IgG4 class/subclass the least
       frequently detected. An unexpected finding was the presence of IgD
       antibodies to HIV-1 proteins in approximately 50% of the individuals.
       The distributions of Ig class/subclass antibodies to the different HIV-1
       proteins were compared in sera from 14 mothers giving birth to infants
       who were determined to be HIV-1 infected with sera from 25 individuals
       whose infants were not infected. Sera from transmitting mothers
       contained a broader distribution of class and subclass antibodies
       compared to sera from nontransmitting women. The single most frequent
       antibody-antigen combination that was found in the transmitting mother
       was IgG2-gp160.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Cohort Studies  Female  Flow Cytometry  Gene Products, env/IMMUNOLOGY
       Human  HIV Antibodies/*BLOOD  HIV Core Protein p24/IMMUNOLOGY  HIV
       Envelope Protein gp120/IMMUNOLOGY  HIV
       Infections/*IMMUNOLOGY/TRANSMISSION  HIV-1/CHEMISTRY/*IMMUNOLOGY
       Immunoglobulins/*BLOOD  Infant, Newborn  Pregnancy  Pregnancy
       Complications, Infectious/*IMMUNOLOGY  Protein Precursors/IMMUNOLOGY
       Reverse Transcriptase/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).